› 1vl0

X-ray diffraction
2.05Å resolution

CRYSTAL STRUCTURE OF A DTDP-4-DEHYDRORHAMNOSE REDUCTASE, RFBD ORTHOLOG (CA_C2315) FROM CLOSTRIDIUM ACETOBUTYLICUM ATCC 824 AT 2.05 A RESOLUTION

Released:
DOI: 10.2210/pdb1vl0/pdb
PDB entry 1vl0 coloured by chain, front view.
PDB entry 1vl0 coloured by chain, side view.
PDB entry 1vl0 coloured by chain, top view.
Source organism: Clostridium acetobutylicum ATCC 824
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
dTDP-beta-L-rhamnose + NADP(+) = dTDP-4-dehydro-beta-L-rhamnose + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188909 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
dTDP-4-dehydrorhamnose reductase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 292 amino acids
Theoretical weight: 33.27 KDa
Source organism: Clostridium acetobutylicum ATCC 824
Expression system: Escherichia coli
UniProt:
  • Canonical: Q97GQ1 (Residues: 1-280; Coverage: 100%)
Gene name: CA_C2315
Sequence domains: RmlD substrate binding domain
Structure domains:

Ligands and Environments


Cofactor: Ligand NAI 3 x NAI
1 bound ligand:
Ligand UNL 1 x UNL
1 modified residue:
Ligand MSE 15 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: C2221
Unit cell:
a: 106.8Å b: 130.67Å c: 151.05Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.169 0.207
Expression system: Escherichia coli

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