› 1vsf

X-ray diffraction
2.05Å resolution

ASV INTEGRASE CORE DOMAIN WITH MN(II) COFACTOR AND HEPES LIGAND, HIGH MG CONCENTRATION FORM

Released:
DOI: 10.2210/pdb1vsf/pdb
PDB entry 1vsf coloured by chain, front view.
PDB entry 1vsf coloured by chain, side view.
PDB entry 1vsf coloured by chain, top view.
Source organism: Rous sarcoma virus (strain Schmidt-Ruppin)
Primary publication:
The catalytic domain of avian sarcoma virus integrase: conformation of the active-site residues in the presence of divalent cations.
Bujacz G, Jaskólski M, Alexandratos J, Wlodawer A, Merkel G, Katz RA, Skalka AM
Structure 4 89-96 (1996)
PMID: 8805516

Function and Biology Details

Reactions catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131654 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Integrase Chain: A
Molecule details ›
Chain: A
Length: 152 amino acids
Theoretical weight: 16.72 KDa
Source organism: Rous sarcoma virus (strain Schmidt-Ruppin)
Expression system: Escherichia coli
UniProt:
  • Canonical: O92956 (Residues: 1333-1479; Coverage: 9%)
Gene name: gag-pol
Sequence domains: Integrase core domain
Structure domains: Ribonuclease H-like superfamily/Ribonuclease H

Ligands and Environments

2 bound ligands:
Ligand MN 1 x MN
Ligand EPE 1 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P43212
Unit cell:
a: 66.24Å b: 66.24Å c: 81.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.13 not available 0.189
Expression system: Escherichia coli

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Citations

10 review citations

Inhibitors of HIV-1 protease: a major success of structure-assisted drug design.
Wlodawer et al. (1998)
9 more

3 mentions without citation

Structural biology of retroviral DNA integration.
Li et al. (2011)
2 more