› 1w0i

X-ray diffraction
2.1Å resolution

Function and Biology Details

Reaction catalysed: 
Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO(2) + [acyl-carrier-protein]
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185250 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-oxoacyl-[acyl-carrier-protein] synthase, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 431 amino acids
Theoretical weight: 45.96 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8L3X9 (Residues: 31-461; Coverage: 94%)
Gene names: At2g04540, KAS, T1O3.5
Sequence domains:
Structure domains: Peroxisomal Thiolase; Chain A, domain 1

Ligands and Environments

2 bound ligands:
Ligand K 2 x K
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: P21
Unit cell:
a: 72.218Å b: 93.246Å c: 74.007Å
α: 90° β: 106.43° γ: 90°
R-values:
R R work R free
0.178 0.178 0.221
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

The thiolase superfamily: condensing enzymes with diverse reaction specificities.
Haapalainen et al. (2006)
1 more

1 mention without citation

X-ray crystal structure of Mycobacterium tuberculosis beta-ketoacyl acyl carrier protein synthase II (mtKasB).
Sridharan et al. (2007)