› 1w17

X-ray diffraction
1.9Å resolution

Structure of Bacillus subtilis PdaA, a family 4 Carbohydrate esterase.

Released:
DOI: 10.2210/pdb1w17/pdb
PDB entry 1w17 coloured by chain, front view.
PDB entry 1w17 coloured by chain, side view.
PDB entry 1w17 coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Structures of Bacillus subtilis PdaA, a family 4 carbohydrate esterase, and a complex with N-acetyl-glucosamine.
Blair DE, van Aalten DM
FEBS Lett 570 13-9 (2004)
PMID: 15251431

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128626 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidoglycan-N-acetylmuramic acid deacetylase PdaA Chains: A, B
Molecule details ›
Chains: A, B
Length: 263 amino acids
Theoretical weight: 30.11 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O34928 (Residues: 1-263; Coverage: 100%)
Gene names: BSU07980, pdaA, yfjS
Sequence domains: Polysaccharide deacetylase
Structure domains: Glycoside hydrolase/deacetylase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P65
Unit cell:
a: 88.115Å b: 88.115Å c: 120.957Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.201 0.201 0.262
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 review citations

Structural variation in the glycan strands of bacterial peptidoglycan.
Vollmer W. (2008)
5 more

7 mentions without citation

Substrate Recognition and Specificity of Chitin Deacetylases and Related Family 4 Carbohydrate Esterases.
Aragunde et al. (2018)
6 more