› 1w1b

X-ray diffraction
2.1Å resolution

Structure of Bacillus subtilis PdaA with Cadmium, a family 4 Carbohydrate esterase.

Released:
DOI: 10.2210/pdb1w1b/pdb
PDB entry 1w1b coloured by chain, front view.
PDB entry 1w1b coloured by chain, side view.
PDB entry 1w1b coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Structures of Bacillus subtilis PdaA, a family 4 carbohydrate esterase, and a complex with N-acetyl-glucosamine.
Blair DE, van Aalten DM
FEBS Lett 570 13-9 (2004)
PMID: 15251431

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128626 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidoglycan-N-acetylmuramic acid deacetylase PdaA Chains: 1, 2
Molecule details ›
Chains: 1, 2
Length: 257 amino acids
Theoretical weight: 29.29 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O34928 (Residues: 10-263; Coverage: 100%)
Gene names: BSU07980, pdaA, yfjS
Sequence domains: Polysaccharide deacetylase
Structure domains: Glycoside hydrolase/deacetylase

Ligands and Environments

1 bound ligand:
Ligand CD 4 x CD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICRO
Spacegroup: P65
Unit cell:
a: 87.824Å b: 87.824Å c: 120.562Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.179 0.179 0.227
Expression system: Escherichia coli BL21(DE3)

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Citations

7 review citations

Structural variation in the glycan strands of bacterial peptidoglycan.
Vollmer W. (2008)
6 more

69 mentions without citation

Structural and chemical biology of deacetylases for carbohydrates, proteins, small molecules and histones.
Bürger et al. (2018)
68 more