› 1w21

X-ray diffraction
2.08Å resolution

Structure of Neuraminidase from English duck subtype N6 complexed with 30 mM sialic acid (NANA, Neu5Ac), crystal soaked for 43 hours at 291 K.

Released:
DOI: 10.2210/pdb1w21/pdb
PDB entry 1w21 coloured by chain, front view.
PDB entry 1w21 coloured by chain, side view.
PDB entry 1w21 coloured by chain, top view.
Source organism: Influenza A virus
Entry authors: Rudino-Pinera E, Tunnah P, Crennell SJ, Webster RG, Laver WG, Garman EF

Function and Biology Details

Reaction catalysed: 
Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180538 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Neuraminidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 389 amino acids
Theoretical weight: 43.01 KDa
Source organism: Influenza A virus
Expression system: Gallus gallus
UniProt:
  • Canonical: Q6XV27 (Residues: 82-470; Coverage: 83%)
Gene name: NA
Sequence domains: Neuraminidase
Structure domains: Neuraminidase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
6 bound ligands:
Ligand SIA 8 x SIA
Ligand GOL 4 x GOL
Ligand NAG 5 x NAG
Ligand BMA 3 x BMA
Ligand MAN 10 x MAN
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200H
Spacegroup: P21
Unit cell:
a: 106.478Å b: 73.995Å c: 106.465Å
α: 90° β: 90.5° γ: 90°
R-values:
R R work R free
0.153 0.15 0.195
Expression system: Gallus gallus

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