› 1w93

X-ray diffraction
2.5Å resolution

Crystal Structure of Biotin Carboxylase Domain of Acetyl-Coenzyme A Carboxylase from Saccharomyces cerevisiae

Released:
DOI: 10.2210/pdb1w93/pdb
PDB entry 1w93 coloured by chain, front view.
PDB entry 1w93 coloured by chain, side view.
PDB entry 1w93 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
A mechanism for the potent inhibition of eukaryotic acetyl-coenzyme A carboxylase by soraphen A, a macrocyclic polyketide natural product.
Shen Y, Volrath SL, Weatherly SC, Elich TD, Tong L
Mol Cell 16 881-91 (2004)
PMID: 15610732

Function and Biology Details

Reactions catalysed: 
ATP + [biotin carboxyl-carrier protein]-biotin-N(6)-L-lysine + hydrogencarbonate- = ADP + phosphate + [biotin carboxyl-carrier protein]-carboxybiotin-N(6)-L-lysine
ATP + acetyl-CoA + HCO(3)(-) = ADP + phosphate + malonyl-CoA
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169487 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetyl-CoA carboxylase Chain: A
Molecule details ›
Chain: A
Length: 553 amino acids
Theoretical weight: 61.52 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q00955 (Residues: 14-566; Coverage: 25%)
Gene names: ABP2, ACC1, FAS3, MTR7, N3175, YNR016C
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P62
Unit cell:
a: 101.74Å b: 101.74Å c: 145.83Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.254 0.254 0.323
Expression system: Escherichia coli

Quick links

Citations

25 review citations

Fatty acid synthesis and elongation in yeast.
Tehlivets et al. (2007)
24 more

1 mention without citation

Molecular Dynamics Scoring of Protein-Peptide Models Derived from Coarse-Grained Docking.
Zalewski et al. (2021)