› 1wa0

X-ray diffraction
1.6Å resolution

Crystal Structure Of W138H Mutant Of Alcaligenes Xylosoxidans Nitrite Reductase

Released:
DOI: 10.2210/pdb1wa0/pdb
PDB entry 1wa0 coloured by chain, front view.
PDB entry 1wa0 coloured by chain, side view.
PDB entry 1wa0 coloured by chain, top view.
Source organism: Achromobacter xylosoxidans
Primary publication:
Insights into redox partner interactions and substrate binding in nitrite reductase from Alcaligenes xylosoxidans: crystal structures of the Trp138His and His313Gln mutants.
Barrett ML, Harris RL, Antonyuk S, Hough MA, Ellis MJ, Sawers G, Eady RR, Hasnain SS
Biochemistry 43 16311-9 (2004)
PMID: 15610025

Function and Biology Details

Reaction catalysed: 
Nitric oxide + H(2)O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130622 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Copper-containing nitrite reductase Chain: X
Molecule details ›
Chain: X
Length: 336 amino acids
Theoretical weight: 36.52 KDa
Source organism: Achromobacter xylosoxidans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O68601 (Residues: 25-360; Coverage: 100%)
Gene names: O9570_16215, nir, nirK
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

5 bound ligands:
Ligand CU 2 x CU
Ligand ZN 2 x ZN
Ligand SO4 1 x SO4
Ligand PG4 1 x PG4
Ligand MES 1 x MES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.1
Spacegroup: R3
Unit cell:
a: 89.792Å b: 89.792Å c: 143.462Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.166 0.165 0.192
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Copper active sites in biology.
Solomon et al. (2014)
3 more