› 1wa3

X-ray diffraction
1.9Å resolution

Mechanism of the Class I KDPG aldolase

Released:
DOI: 10.2210/pdb1wa3/pdb
PDB entry 1wa3 coloured by chain, front view.
PDB entry 1wa3 coloured by chain, side view.
PDB entry 1wa3 coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
Mechanism of the Class I KDPG aldolase.
Fullerton SW, Griffiths JS, Merkel AB, Cheriyan M, Wymer NJ, Hutchins MJ, Fierke CA, Toone EJ, Naismith JH
Bioorg Med Chem 14 3002-10 (2006)
PMID: 16403639

Function and Biology Details

Reaction catalysed: 
2-dehydro-3-deoxy-6-phosphate-D-gluconate = pyruvate + D-glyceraldehyde 3-phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194435 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 205 amino acids
Theoretical weight: 22.27 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9WXS1 (Residues: 1-205; Coverage: 100%)
Gene name: TM_0066
Sequence domains: KDPG and KHG aldolase
Structure domains: Aldolase class I

Ligands and Environments

2 bound ligands:
Ligand PYR 6 x PYR
Ligand SO4 6 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 42.6Å b: 101.1Å c: 124.6Å
α: 90° β: 97.2° γ: 90°
R-values:
R R work R free
0.195 0.193 0.239
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Carbohydrate metabolism in Archaea: current insights into unusual enzymes and pathways and their regulation.
Bräsen et al. (2014)
4 more

12 mentions without citation

Structural and biochemical studies of human 4-hydroxy-2-oxoglutarate aldolase: implications for hydroxyproline metabolism in primary hyperoxaluria.
Riedel et al. (2011)
11 more