› 1wdy

X-ray diffraction
1.8Å resolution

Crystal structure of ribonuclease

Released:
DOI: 10.2210/pdb1wdy/pdb
PDB entry 1wdy coloured by chain, front view.
PDB entry 1wdy coloured by chain, side view.
PDB entry 1wdy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for recognition of 2',5'-linked oligoadenylates by human ribonuclease L.
Tanaka N, Nakanishi M, Kusakabe Y, Goto Y, Kitade Y, Nakamura KT
EMBO J 23 3929-38 (2004)
PMID: 15385955

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170254 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2-5A-dependent ribonuclease Chain: A
Molecule details ›
Chain: A
Length: 285 amino acids
Theoretical weight: 31.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q05823 (Residues: 21-305; Coverage: 39%)
Gene names: RNASEL, RNS4
Sequence domains: Ankyrin repeat
Structure domains: Ankyrin repeat-containing domain

Ligands and Environments

1 bound ligand:
Ligand 25A 1 x 25A
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P212121
Unit cell:
a: 63.203Å b: 72.834Å c: 82.634Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.202 0.23
Expression system: Escherichia coli

Quick links

Citations

19 review citations

Viral encounters with 2',5'-oligoadenylate synthetase and RNase L during the interferon antiviral response.
Silverman RH. (2007)
18 more

5 mentions without citation

A primer on ankyrin repeat function in TRP channels and beyond.
Gaudet R. (2008)
4 more