› 1wg3

X-ray diffraction
3Å resolution

Structural analysis of yeast nucleosome-assembly factor CIA1p

Released:
DOI: 10.2210/pdb1wg3/pdb
PDB entry 1wg3 coloured by chain, front view.
PDB entry 1wg3 coloured by chain, side view.
PDB entry 1wg3 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structural similarity between histone chaperone Cia1p/Asf1p and DNA-binding protein NF-kappaB.
Padmanabhan B, Kataoka K, Umehara T, Adachi N, Yokoyama S, Horikoshi M
J Biochem 138 821-9 (2005)
PMID: 16428312

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152304 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Histone chaperone ASF1 Chain: A
Molecule details ›
Chain: A
Length: 175 amino acids
Theoretical weight: 19.59 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P32447 (Residues: 1-169; Coverage: 61%)
Gene names: ASF1, CIA1, J0755, YJL115W
Sequence domains: ASF1 like histone chaperone
Structure domains: Histone chaperone ASF1-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-18B
Spacegroup: P21212
Unit cell:
a: 106.629Å b: 47.048Å c: 40.686Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.198 0.274
Expression system: Escherichia coli

Quick links

Citations

2 review citations

The histone chaperone Asf1 at the crossroads of chromatin and DNA checkpoint pathways.
Mousson et al. (2007)
1 more

1 mention without citation

Structural basis for the histone chaperone activity of Asf1.
English et al. (2006)