› 1wnu

X-ray diffraction
2.8Å resolution

Structure of Archaeal Trans-Editing Protein AlaX in complex with L-serine

Released:
DOI: 10.2210/pdb1wnu/pdb
PDB entry 1wnu coloured by chain, front view.
PDB entry 1wnu coloured by chain, side view.
PDB entry 1wnu coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Primary publication:
Molecular basis of alanine discrimination in editing site.
Sokabe M, Okada A, Yao M, Nakashima T, Tanaka I
Proc Natl Acad Sci U S A 102 11669-74 (2005)
PMID: 16087889

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129702 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alanyl-tRNA editing protein AlaX-S Chains: A, B
Molecule details ›
Chains: A, B
Length: 165 amino acids
Theoretical weight: 19.39 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
UniProt:
  • Canonical: O58307 (Residues: 1-157; Coverage: 100%)
Gene names: PH0574, alaXS
Sequence domains: Threonyl and Alanyl tRNA synthetase second additional domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand SER 2 x SER
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P212121
Unit cell:
a: 33.968Å b: 88.472Å c: 109.921Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.228 0.266
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Aminoacyl-tRNA synthesis and translational quality control.
Ling et al. (2009)
5 more

6 mentions without citation

Computational design of high-affinity epitope scaffolds by backbone grafting of a linear epitope.
Azoitei et al. (2012)
5 more