› 1wx2

X-ray diffraction
1.8Å resolution

Crystal Structure of the oxy-form of the copper-bound Streptomyces castaneoglobisporus tyrosinase complexed with a caddie protein prepared by the addition of hydrogenperoxide

Released:
DOI: 10.2210/pdb1wx2/pdb
PDB entry 1wx2 coloured by chain, front view.
PDB entry 1wx2 coloured by chain, side view.
PDB entry 1wx2 coloured by chain, top view.
Source organism: Streptomyces castaneoglobisporus
Primary publication:
Crystallographic evidence that the dinuclear copper center of tyrosinase is flexible during catalysis.
Matoba Y, Kumagai T, Yamamoto A, Yoshitsu H, Sugiyama M
J Biol Chem 281 8981-90 (2006)
PMID: 16436386

Function and Biology Details

Reaction catalysed: 
(1a) L-tyrosine + 1/2 O(2) = L-dopa
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182976 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosinase copper-binding domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 281 amino acids
Theoretical weight: 32.09 KDa
Source organism: Streptomyces castaneoglobisporus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q83WS2 (Residues: 1-273; Coverage: 100%)
Sequence domains: Common central domain of tyrosinase
Structure domains: Di-copper center containing domain from catechol oxidase
MelC Chain: B
Molecule details ›
Chain: B
Length: 134 amino acids
Theoretical weight: 14.2 KDa
Source organism: Streptomyces castaneoglobisporus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q83WS1 (Residues: 1-126; Coverage: 100%)
Sequence domains: Tyrosinase co-factor MelC1
Structure domains: protein ne1242 domain like

Ligands and Environments

3 bound ligands:
Ligand CU 4 x CU
Ligand NO3 4 x NO3
Ligand PER 1 x PER
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P21212
Unit cell:
a: 65.16Å b: 96.55Å c: 54.72Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.21 0.249
Expression system: Escherichia coli

Quick links

Citations

41 review citations

An updated review of tyrosinase inhibitors.
Chang TS. (2009)
40 more

25 mentions without citation

Copper active sites in biology.
Solomon et al. (2014)
24 more