› 1wxh

X-ray diffraction
1.9Å resolution

E.coli NAD Synthetase, NAD

Released:
DOI: 10.2210/pdb1wxh/pdb
PDB entry 1wxh coloured by chain, front view.
PDB entry 1wxh coloured by chain, side view.
PDB entry 1wxh coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structures of Escherichia coli NAD synthetase with substrates and products reveal mechanistic rearrangements.
Jauch R, Humm A, Huber R, Wahl MC
J Biol Chem 280 15131-40 (2005)
PMID: 15699042

Function and Biology Details

Reaction catalysed: 
ATP + deamido-NAD(+) + NH(3) = AMP + diphosphate + NAD(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-148453 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NH(3)-dependent NAD(+) synthetase Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 30.67 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P18843 (Residues: 1-275; Coverage: 100%)
Gene names: JW1729, b1740, efg, nadE, ntrL
Sequence domains: NAD synthase
Structure domains: HUPs

Ligands and Environments

1 bound ligand:
Ligand NAD 1 x NAD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P3121
Unit cell:
a: 92.239Å b: 92.239Å c: 76.239Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.171 0.171 0.191
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Nicotinamide adenine dinucleotide metabolism as an attractive target for drug discovery.
Khan et al. (2007)
1 more

3 mentions without citation

Autonomous aggregation suppression by acidic residues explains why chaperones favour basic residues.
Houben et al. (2020)
2 more