› 1x0c

X-ray diffraction
1.7Å resolution

Improved Crystal Structure of Isopullulanase from Aspergillus niger ATCC 9642

Released:
DOI: 10.2210/pdb1x0c/pdb
PDB entry 1x0c coloured by chain, front view.
PDB entry 1x0c coloured by chain, side view.
PDB entry 1x0c coloured by chain, top view.
Source organism: Aspergillus niger
Primary publication:
Crystal structure of Aspergillus niger isopullulanase, a member of glycoside hydrolase family 49.
Mizuno M, Koide A, Yamamura A, Akeboshi H, Yoshida H, Kamitori S, Sakano Y, Nishikawa A, Tonozuka T
J Mol Biol 376 210-20 (2008)
PMID: 18155243

Function and Biology Details

Reaction catalysed: 
Hydrolysis of pullulan to isopanose (6-alpha-maltosylglucose)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126117 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isopullulanase Chains: A, B
Molecule details ›
Chains: A, B
Length: 549 amino acids
Theoretical weight: 60.04 KDa
Source organism: Aspergillus niger
Expression system: Komagataella pastoris
UniProt:
  • Canonical: O00105 (Residues: 20-564; Coverage: 100%)
Gene name: ipuA
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand NAG 23 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P212121
Unit cell:
a: 99.725Å b: 136.158Å c: 84.473Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.195 0.229
Expression system: Komagataella pastoris

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Citations

1 review citation

Characteristics, protein engineering and applications of microbial thermostable pullulanases and pullulan hydrolases.
Nisha et al. (2016)
7 other articles