1x1i

X-ray diffraction
1.8Å resolution

Crystal Structure of Xanthan Lyase (N194A) Complexed with a Product

Released:

Function and Biology Details

Reaction catalysed:
Eliminative cleavage of the terminal beta-D-mannosyl-(1->4)-beta-D-glucuronosyl linkage of the side-chain of the polysaccharide xanthan, leaving a 4-deoxy-alpha-L-threo-hex-4-enuronosyl group at the terminus of the side-chain
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189600 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Xanthan lyase Chain: A
Molecule details ›
Chain: A
Length: 752 amino acids
Theoretical weight: 80.71 KDa
Source organism: Bacillus sp. GL1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9AQS0 (Residues: 26-777; Coverage: 83%)
Gene name: xly
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P21
Unit cell:
a: 53.802Å b: 90.677Å c: 77.92Å
α: 90° β: 99.09° γ: 90°
R-values:
R R work R free
0.176 0.176 0.204
Expression system: Escherichia coli