› 1x8z

X-ray diffraction
2.86Å resolution

Crystal structure of a pectin methylesterase inhibitor from Arabidopsis thaliana

Released:
DOI: 10.2210/pdb1x8z/pdb
PDB entry 1x8z coloured by chain, front view.
PDB entry 1x8z coloured by chain, side view.
PDB entry 1x8z coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Structural insights into the target specificity of plant invertase and pectin methylesterase inhibitory proteins.
Hothorn M, Wolf S, Aloy P, Greiner S, Scheffzek K
Plant Cell 16 3437-47 (2004)
PMID: 15528298

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo trimer
Assembly name:
PDBe Complex ID:
PDB-CPX-192296 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pectinesterase inhibitor 1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 153 amino acids
Theoretical weight: 16.44 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9LNF2 (Residues: 27-176; Coverage: 99%)
Gene names: At1g48020, F21D18.29, F21D18_23, PMEI1, T2J15.7
Sequence domains: Plant invertase/pectin methylesterase inhibitor
Structure domains: Invertase/pectin methylesterase inhibitor family protein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: I222
Unit cell:
a: 60.77Å b: 106.19Å c: 186.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.218 0.28
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Structural systems biology: modelling protein interactions.
Aloy et al. (2006)
11 more

5 mentions without citation

Tuning of Pectin Methylesterification: PECTIN METHYLESTERASE INHIBITOR 7 MODULATES THE PROCESSIVE ACTIVITY OF CO-EXPRESSED PECTIN METHYLESTERASE 3 IN A pH-DEPENDENT MANNER.
Sénéchal et al. (2015)
4 more