› 1xdj

X-ray diffraction
2.2Å resolution

Crystal Structure of T. maritima Cobalamin-Independent Methionine Synthase complexed with Zn2+ and Homocysteine

Released:
DOI: 10.2210/pdb1xdj/pdb
PDB entry 1xdj coloured by chain, front view.
PDB entry 1xdj coloured by chain, side view.
PDB entry 1xdj coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
Cobalamin-independent methionine synthase (MetE): a face-to-face double barrel that evolved by gene duplication.
Pejchal R, Ludwig ML
OpenAccess logo PLoS Biol 3 e31 (2005)
PMID: 15630480

Function and Biology Details

Reaction catalysed: 
5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194762 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 766 amino acids
Theoretical weight: 90.14 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9X112 (Residues: 2-734; Coverage: 100%)
Gene names: TM_1286, metE
Sequence domains:
Structure domains: TIM Barrel

Ligands and Environments

4 bound ligands:
Ligand SO4 2 x SO4
Ligand ZN 2 x ZN
Ligand HCS 2 x HCS
Ligand MRY 2 x MRY
1 modified residue:
Ligand MSE 34 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: P21212
Unit cell:
a: 167.053Å b: 159.366Å c: 65.639Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.247 0.227 0.268
Expression system: Escherichia coli

Quick links

Citations

9 review citations

The structural and functional diversity of metabolite-binding riboswitches.
Roth et al. (2009)
8 more

2 mentions without citation

Cobalamin-independent methionine synthase (MetE): a face-to-face double barrel that evolved by gene duplication.
Pejchal et al. (2005)
1 more