› 1xh2

X-ray diffraction
2.2Å resolution

Structure of the N298S variant of human pancreatic alpha-amylase complexed with chloride and acarbose

Released:
DOI: 10.2210/pdb1xh2/pdb
PDB entry 1xh2 coloured by chain, front view.
PDB entry 1xh2 coloured by chain, side view.
PDB entry 1xh2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and mechanistic studies of chloride induced activation of human pancreatic alpha-amylase.
Maurus R, Begum A, Kuo HH, Racaza A, Numao S, Andersen C, Tams JW, Vind J, Overall CM, Withers SG, Brayer GD
Protein Sci 14 743-55 (2005)
PMID: 15722449

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138163 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pancreatic alpha-amylase Chain: A
Molecule details ›
Chain: A
Length: 496 amino acids
Theoretical weight: 55.9 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P04746 (Residues: 16-511; Coverage: 100%)
Gene name: AMY2A
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand NAG 1 x NAG
Ligand ARE 1 x ARE
Ligand CA 1 x CA
Ligand CL 1 x CL
1 modified residue:
Ligand PCA 1 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P212121
Unit cell:
a: 53.135Å b: 69.392Å c: 132.447Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.156 0.214
Expression system: Komagataella pastoris

Quick links

Citations

2 review citations

The nutraceutical role of the Phaseolus vulgaris alpha-amylase inhibitor.
Obiro et al. (2008)
1 more