› 1xj0

X-ray diffraction
1.7Å resolution

Crystal Structure of the GDP-bound form of the RasG60A mutant

Released:
DOI: 10.2210/pdb1xj0/pdb
PDB entry 1xj0 coloured by chain, front view.
PDB entry 1xj0 coloured by chain, side view.
PDB entry 1xj0 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Nassar N, Skowronek K

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133956 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GTPase HRas, N-terminally processed Chain: A
Molecule details ›
Chain: A
Length: 166 amino acids
Theoretical weight: 18.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P01112 (Residues: 1-166; Coverage: 88%)
Gene names: HRAS, HRAS1
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand GDP 1 x GDP
1 modified residue:
Ligand CSX 1 x CSX

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: R32
Unit cell:
a: 93.64Å b: 93.64Å c: 121.56Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.228 0.228 0.239
Expression system: Escherichia coli BL21

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