Function and Biology Details
Biochemical function:
Biological process:
Cellular component:
Sequence domain:
Structure domain:
Structure analysis Details
Assembly composition:
monomeric (preferred)
Assembly name:
Zinc-dependent sulfurtransferase SufU (preferred)
PDBe Complex ID:
PDB-CPX-128423 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ligands and Environments
1 bound ligand:
No modified residues
Experiments and Validation Details
Refinement method:
Minimal constraint structure contained 492 conformationally restricting NOE-derived distance constraints, 108 hydrogen bond constraints, and 197 dihedral angle constraints. This resulted in 5.9 constraints per residue and 1.2 long range constraints per residue. Structure determination was performed
with the following steps:
AutoStructure-Dyana was used to identify distance constraints. These distance constraints were used as input into a Simulated Annealing with Xplor-NIH. The top ten structures were energy minimized with water using CNS.
Expression system: Escherichia coli