› 1xsi

X-ray diffraction
2.2Å resolution

Structure of a Family 31 alpha glycosidase

Released:
DOI: 10.2210/pdb1xsi/pdb
PDB entry 1xsi coloured by chain, front view.
PDB entry 1xsi coloured by chain, side view.
PDB entry 1xsi coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Mechanistic and structural analysis of a family 31 alpha-glycosidase and its glycosyl-enzyme intermediate.
Lovering AL, Lee SS, Kim YW, Withers SG, Strynadka NC
J Biol Chem 280 2105-15 (2005)
PMID: 15501829

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal, non-reducing alpha-D-xylose residues with release of alpha-D-xylose.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152013 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-xylosidase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 778 amino acids
Theoretical weight: 89 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P31434 (Residues: 1-772; Coverage: 100%)
Gene names: JW3631, b3656, yicI
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand SO4 18 x SO4
Ligand ACY 3 x ACY
Ligand MPO 4 x MPO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P212121
Unit cell:
a: 161.686Å b: 174.926Å c: 209.509Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.2 0.239
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Mechanistic insights into glycosidase chemistry.
Vocadlo et al. (2008)
10 more

3 mentions without citation

Detection of trans-cis flips and peptide-plane flips in protein structures.
Touw et al. (2015)
2 more