› 1xtv

X-ray diffraction
2.6Å resolution

Sulfolobus solfataricus uracil phosphoribosyltransferase with uridine 5'-monophosphate (UMP) bound to half of the subunits

Released:
DOI: 10.2210/pdb1xtv/pdb
PDB entry 1xtv coloured by chain, front view.
PDB entry 1xtv coloured by chain, side view.
PDB entry 1xtv coloured by chain, top view.
Source organism: Saccharolobus solfataricus
Primary publication:
Allosteric regulation and communication between subunits in uracil phosphoribosyltransferase from Sulfolobus solfataricus.
Arent S, Harris P, Jensen KF, Larsen S
Biochemistry 44 883-92 (2005)
PMID: 15654744

Function and Biology Details

Reaction catalysed: 
UMP + diphosphate = uracil + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189097 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uracil phosphoribosyltransferase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 216 amino acids
Theoretical weight: 24.18 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q980Q4 (Residues: 1-216; Coverage: 100%)
Gene names: SSO0231, upp
Sequence domains: Uracil phosphoribosyltransferase
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand U5P 4 x U5P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: P1
Unit cell:
a: 68.1Å b: 73.57Å c: 115.1Å
α: 85.82° β: 85.28° γ: 62.46°
R-values:
R R work R free
0.227 0.227 0.263
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Phosphoribosyl Diphosphate (PRPP): Biosynthesis, Enzymology, Utilization, and Metabolic Significance.
Hove-Jensen et al. (2017)
1 more

2 mentions without citation

Approximate subgraph matching-based literature mining for biomedical events and relations.
Liu et al. (2013)
1 more