› 1xzk

X-ray diffraction
2.01Å resolution

FUSARIUM SOLANI CUTINASE COMPLEX WITH DI(ISOPROPYL)PHOSPHATE

Released:
DOI: 10.2210/pdb1xzk/pdb
PDB entry 1xzk coloured by chain, front view.
PDB entry 1xzk coloured by chain, side view.
PDB entry 1xzk coloured by chain, top view.
Source organism: Fusarium vanettenii
Primary publication:
Dynamics of Fusarium solani cutinase investigated through structural comparison among different crystal forms of its variants.
Longhi S, Nicolas A, Creveld L, Egmond M, Verrips CT, de Vlieg J, Martinez C, Cambillau C
Proteins 26 442-58 (1996)
PMID: 8990497

Function and Biology Details

Reaction catalysed: 
Cutin + H(2)O = cutin monomers
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132745 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cutinase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 214 amino acids
Theoretical weight: 22.35 KDa
Source organism: Fusarium vanettenii
Expression system: Escherichia coli
UniProt:
  • Canonical: P00590 (Residues: 17-230; Coverage: 100%)
Gene names: CUT1, CUTA
Sequence domains: Cutinase
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand DFP 2 x DFP
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 37.2Å b: 94.8Å c: 55Å
α: 90° β: 101.3° γ: 90°
R-values:
R R work R free
0.161 0.161 not available
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Cutinase: from molecular level to bioprocess development.
Carvalho et al. (1999)
2 more

2 mentions without citation

Perspectives on the Role of Enzymatic Biocatalysis for the Degradation of Plastic PET.
Magalhães et al. (2021)
1 more