› 1y19

X-ray diffraction
2.6Å resolution

Structural basis for phosphatidylinositol phosphate kinase type I-gamma binding to talin at focal adhesions

Released:
DOI: 10.2210/pdb1y19/pdb
PDB entry 1y19 coloured by chain, front view.
PDB entry 1y19 coloured by chain, side view.
PDB entry 1y19 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural basis for phosphatidylinositol phosphate kinase type Igamma binding to talin at focal adhesions.
de Pereda JM, Wegener KL, Santelli E, Bate N, Ginsberg MH, Critchley DR, Campbell ID, Liddington RC
J Biol Chem 280 8381-6 (2005)
PMID: 15623515

Function and Biology Details

Reaction catalysed: 
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130676 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Phosphatidylinositol 4-phosphate 5-kinase type-1 gamma Chains: A, C, E, G, I, K
Molecule details ›
Chains: A, C, E, G, I, K
Length: 14 amino acids
Theoretical weight: 1.71 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O70161 (Residues: 638-651; Coverage: 2%)
Gene names: Kiaa0589, Pip5k1c
Talin-1 Chains: B, D, F, H, J, L
Molecule details ›
Chains: B, D, F, H, J, L
Length: 202 amino acids
Theoretical weight: 23.28 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P26039 (Residues: 209-410; Coverage: 8%)
Gene names: Tln, Tln1
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL1-5
Spacegroup: P32
Unit cell:
a: 118.102Å b: 118.102Å c: 93.308Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.253 0.253 0.286
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

16 review citations

Talins and kindlins: partners in integrin-mediated adhesion.
Calderwood et al. (2013)
15 more

3 mentions without citation

Targeting Pyk2 for therapeutic intervention.
Lipinski et al. (2010)
2 more