› 1yc8

X-ray diffraction
2.7Å resolution

cAbAn33- Y37V/E44G/R45L triple mutant

Released:
DOI: 10.2210/pdb1yc8/pdb
PDB entry 1yc8 coloured by chain, front view.
PDB entry 1yc8 coloured by chain, side view.
PDB entry 1yc8 coloured by chain, top view.
Source organism: Camelus dromedarius
Primary publication:
Antigen binding and solubility effects upon the veneering of a camel VHH in framework-2 to mimic a VH.
Conrath K, Vincke C, Stijlemans B, Schymkowitz J, Decanniere K, Wyns L, Muyldermans S, Loris R
J Mol Biol 350 112-25 (2005)
PMID: 15913651

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-213119 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
anti-VSG immunoglobulin heavy chain variable domain cAbAn33 Chains: A, B
Molecule details ›
Chains: A, B
Length: 124 amino acids
Theoretical weight: 13.62 KDa
Source organism: Camelus dromedarius
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P43212
Unit cell:
a: 51.843Å b: 51.843Å c: 164.705Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.253 0.246 0.324
Expression system: Escherichia coli

Quick links

Citations

22 review citations

Engineered antibody fragments and the rise of single domains.
Holliger et al. (2005)
21 more

2 mentions without citation

Discrete analysis of camelid variable domains: sequences, structures, and in-silico structure prediction.
Melarkode Vattekatte et al. (2020)
1 more