› 1ylj

X-ray diffraction
0.98Å resolution

Atomic resolution structure of CTX-M-9 beta-lactamase

Released:
DOI: 10.2210/pdb1ylj/pdb
PDB entry 1ylj coloured by chain, front view.
PDB entry 1ylj coloured by chain, side view.
PDB entry 1ylj coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Atomic resolution structures of CTX-M beta-lactamases: extended spectrum activities from increased mobility and decreased stability.
Chen Y, Delmas J, Sirot J, Shoichet B, Bonnet R
J Mol Biol 348 349-62 (2005)
PMID: 15811373

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192212 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Beta-lactamase Chain: A
Molecule details ›
Chain: A
Length: 263 amino acids
Theoretical weight: 27.97 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9L5C8 (Residues: 29-291; Coverage: 100%)
Gene names: RCS51_P0174, bla CTX-M-9, blaCTX-M-9, blaCTX-M-9a, blaCTX-M-9b
Sequence domains:
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, FRU
Carbohydrate polymer
1 bound ligand:
Ligand SO4 10 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 41.388Å b: 62.236Å c: 85.746Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.108 0.108 0.129
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

18 review citations

Three decades of beta-lactamase inhibitors.
Drawz et al. (2010)
17 more

14 mentions without citation

Distant and new mutations in CTX-M-1 beta-lactamase affect cefotaxime hydrolysis.
Pérez-Llarena et al. (2011)
13 more