› 1yuj

Solution NMR

SOLUTION NMR STRUCTURE OF THE GAGA FACTOR/DNA COMPLEX, 50 STRUCTURES

Released:
DOI: 10.2210/pdb1yuj/pdb
PDB entry 1yuj coloured by chain, ensemble of 50 models, front view.
PDB entry 1yuj coloured by chain, ensemble of 50 models, side view.
PDB entry 1yuj coloured by chain, ensemble of 50 models, top view.
Source organism: Drosophila melanogaster
Primary publication:
The solution structure of a specific GAGA factor-DNA complex reveals a modular binding mode.
Omichinski JG, Pedone PV, Felsenfeld G, Gronenborn AM, Clore GM
Nat Struct Biol 4 122-32 (1997)
PMID: 9033593

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114875 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Transcription activator GAGA Chain: A
Molecule details ›
Chain: A
Length: 54 amino acids
Theoretical weight: 6.2 KDa
Source organism: Drosophila melanogaster
UniProt:
  • Canonical: Q08605 (Residues: 319-372; Coverage: 9%)
Gene names: Adf-2, CG33261, GAGA, Nc70F, TFGAGA, Trl
Sequence domains: GAGA factor
Structure domains: Classic Zinc Finger
DNA (5'-D(*GP*CP*CP*GP*AP*GP*AP*GP*TP*AP*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 11 nucleotides
Theoretical weight: 3.38 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided
DNA (5'-D(*GP*TP*AP*CP*TP*CP*TP*CP*GP*GP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 11 nucleotides
Theoretical weight: 3.33 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Not provided

Quick links

Citations

18 review citations

DNA recognition by Cys2His2 zinc finger proteins.
Wolfe et al. (2000)
17 more

4 mentions without citation

Conservation of the three-dimensional structure in non-homologous or unrelated proteins.
Sousounis et al. (2012)
3 more