› 1yvn

X-ray diffraction
2.1Å resolution

THE YEAST ACTIN VAL 159 ASN MUTANT COMPLEX WITH HUMAN GELSOLIN SEGMENT 1.

Released:
DOI: 10.2210/pdb1yvn/pdb
PDB entry 1yvn coloured by chain, front view.
PDB entry 1yvn coloured by chain, side view.
PDB entry 1yvn coloured by chain, top view.
Source organisms:
Entry authors: Vorobiev SM, Belmont LD, Drubin DG, Almo SC

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-139013 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Actin Chain: A
Molecule details ›
Chain: A
Length: 375 amino acids
Theoretical weight: 41.75 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P60010 (Residues: 1-375; Coverage: 100%)
Gene names: ABY1, ACT1, END7, YFL039C
Sequence domains: Actin
Structure domains:
Gelsolin Chain: G
Molecule details ›
Chain: G
Length: 125 amino acids
Theoretical weight: 14.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06396 (Residues: 52-176; Coverage: 17%)
Gene name: GSN
Sequence domains: Gelsolin repeat
Structure domains: Severin

Ligands and Environments

4 bound ligands:
Ligand MG 1 x MG
Ligand SO4 1 x SO4
Ligand ATP 1 x ATP
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: C2
Unit cell:
a: 176.098Å b: 68.077Å c: 54.691Å
α: 90° β: 102.24° γ: 90°
R-values:
R R work R free
0.187 0.187 0.235
Expression system: Escherichia coli BL21(DE3)

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