› 1yzz

X-ray diffraction
2.7Å resolution

Humanized caban33 at room temperature

Released:
DOI: 10.2210/pdb1yzz/pdb
PDB entry 1yzz coloured by chain, front view.
PDB entry 1yzz coloured by chain, side view.
PDB entry 1yzz coloured by chain, top view.
Source organism: Camelus dromedarius
Primary publication:
Antigen binding and solubility effects upon the veneering of a camel VHH in framework-2 to mimic a VH.
Conrath K, Vincke C, Stijlemans B, Schymkowitz J, Decanniere K, Wyns L, Muyldermans S, Loris R
J Mol Biol 350 112-25 (2005)
PMID: 15913651

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-213122 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
anti-VSG immunoglobulin heavy chain variable domain cAbAn33 Chains: A, B
Molecule details ›
Chains: A, B
Length: 124 amino acids
Theoretical weight: 13.62 KDa
Source organism: Camelus dromedarius
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P43212
Unit cell:
a: 52.473Å b: 52.473Å c: 165.365Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.233 0.293
Expression system: Escherichia coli

Quick links

Citations

24 review citations

Nanobodies: natural single-domain antibodies.
Muyldermans S. (2013)
23 more