› 1z3j

Solution NMR

Solution Structure of MMP12 in the presence of N-isobutyl-N-4-methoxyphenylsulfonyl]glycyl hydroxamic acid (NNGH)

Released:
DOI: 10.2210/pdb1z3j/pdb
PDB entry 1z3j coloured by chain, front view.
PDB entry 1z3j coloured by chain, side view.
PDB entry 1z3j coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Conformational variability of matrix metalloproteinases: beyond a single 3D structure.
Bertini I, Calderone V, Cosenza M, Fragai M, Lee YM, Luchinat C, Mangani S, Terni B, Turano P
Proc Natl Acad Sci U S A 102 5334-9 (2005)
PMID: 15809432

Function and Biology Details

Reaction catalysed: 
Hydrolysis of soluble and insoluble elastin (1). Specific cleavages are also produced at -Ala(14)-|-Leu- and -Tyr(16)-|-Leu- in the B chain of insulin (2).
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153971 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Macrophage metalloelastase Chain: A
Molecule details ›
Chain: A
Length: 159 amino acids
Theoretical weight: 17.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P39900 (Residues: 106-263; Coverage: 35%)
Gene names: HME, MMP12
Sequence domains: Matrixin
Structure domains: Collagenase (Catalytic Domain)

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand CA 3 x CA
Ligand NGH 1 x NGH
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: distance geometry, simulated annealing, torsion angle dynamics, residue dipolar coupling
Expression system: Escherichia coli

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Citations

14 review citations

Tumour microenvironment - opinion: validating matrix metalloproteinases as drug targets and anti-targets for cancer therapy.
Overall et al. (2006)
13 more

1 mention without citation

Conformational variability of matrix metalloproteinases: beyond a single 3D structure.
Bertini et al. (2005)