› 1zcp

X-ray diffraction
2.3Å resolution

Crystal Structure of a catalytic site mutant E. coli TrxA (CACA)

Released:
DOI: 10.2210/pdb1zcp/pdb
PDB entry 1zcp coloured by chain, front view.
PDB entry 1zcp coloured by chain, side view.
PDB entry 1zcp coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The crystal structure of TrxA(CACA): Insights into the formation of a [2Fe-2S] iron-sulfur cluster in an Escherichia coli thioredoxin mutant.
Collet JF, Peisach D, Bardwell JC, Xu Z
Protein Sci 14 1863-9 (2005)
PMID: 15987909

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141961 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thioredoxin 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 108 amino acids
Theoretical weight: 11.68 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AA25 (Residues: 2-109; Coverage: 99%)
Gene names: JW5856, b3781, fipA, trxA, tsnC
Sequence domains: Thioredoxin
Structure domains: Glutaredoxin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: P21
Unit cell:
a: 34.72Å b: 46.25Å c: 126Å
α: 90° β: 93.13° γ: 90°
R-values:
R R work R free
0.229 0.229 0.281
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Iron-sulfur protein folds, iron-sulfur chemistry, and evolution.
Meyer J. (2008)
2 more

1 mention without citation

The crystal structure of TrxA(CACA): Insights into the formation of a [2Fe-2S] iron-sulfur cluster in an Escherichia coli thioredoxin mutant.
Collet et al. (2005)