› 1zgo

X-ray diffraction
1.4Å resolution

High Resolution Crystal Structure of the Discosoma Red Fluorescent Protein (DsRed)

Released:
DOI: 10.2210/pdb1zgo/pdb
PDB entry 1zgo coloured by chain, front view.
PDB entry 1zgo coloured by chain, side view.
PDB entry 1zgo coloured by chain, top view.
Source organism: Discosoma sp.
Primary publication:
Crystallographic structures of Discosoma red fluorescent protein with immature and mature chromophores: linking peptide bond trans-cis isomerization and acylimine formation in chromophore maturation.
Tubbs JL, Tainer JA, Getzoff ED
Biochemistry 44 9833-40 (2005)
PMID: 16026155

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193624 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Red fluorescent protein drFP583 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 223 amino acids
Theoretical weight: 25.95 KDa
Source organism: Discosoma sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9U6Y8 (Residues: 1-225; Coverage: 100%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CRQ 4 x CRQ

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: P21
Unit cell:
a: 55.717Å b: 127.221Å c: 57.118Å
α: 90° β: 100.42° γ: 90°
R-values:
R R work R free
0.143 0.143 0.206
Expression system: Escherichia coli

Quick links

Citations

3 review citations

The family of GFP-like proteins: structure, function, photophysics and biosensor applications. Introduction and perspective.
Wachter RM. (2006)
2 more

9 mentions without citation

Chromophore transformations in red fluorescent proteins.
Subach et al. (2012)
8 more