› 1zjr

X-ray diffraction
1.85Å resolution

Crystal Structure of A. aeolicus TrmH/SpoU tRNA modifying enzyme

Released:
DOI: 10.2210/pdb1zjr/pdb
PDB entry 1zjr coloured by chain, front view.
PDB entry 1zjr coloured by chain, side view.
PDB entry 1zjr coloured by chain, top view.
Source organism: Aquifex aeolicus
Primary publication:
Structure of a class II TrmH tRNA-modifying enzyme from Aquifex aeolicus.
Pleshe E, Truesdell J, Batey RT
Acta Crystallogr Sect F Struct Biol Cryst Commun 61 722-8 (2005)
PMID: 16511140

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + guanosine(18) in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylguanosine(18) in tRNA
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-130514 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA (guanosine(18)-2'-O)-methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 211 amino acids
Theoretical weight: 24.53 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O67577 (Residues: 1-211; Coverage: 100%)
Gene names: aq_1661, spoU, trmH
Sequence domains:
Structure domains: Alpha/beta knot

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P6122
Unit cell:
a: 66.658Å b: 66.658Å c: 194.884Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.237 0.216 0.249
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Methylated nucleosides in tRNA and tRNA methyltransferases.
Hori H. (2014)
4 more

3 mentions without citation

Structural and evolutionary bioinformatics of the SPOUT superfamily of methyltransferases.
Tkaczuk et al. (2007)
2 more