› 1zlh

X-ray diffraction
1.7Å resolution

Crystal structure of the tick carboxypeptidase inhibitor in complex with bovine carboxypeptidase A

Released:
DOI: 10.2210/pdb1zlh/pdb
PDB entry 1zlh coloured by chain, front view.
PDB entry 1zlh coloured by chain, side view.
PDB entry 1zlh coloured by chain, top view.
Source organisms:
Primary publication:
The three-dimensional structures of tick carboxypeptidase inhibitor in complex with A/B carboxypeptidases reveal a novel double-headed binding mode.
Arolas JL, Popowicz GM, Lorenzo J, Sommerhoff CP, Huber R, Aviles FX, Holak TA
J Mol Biol 350 489-98 (2005)
PMID: 15961103

Function and Biology Details

Reaction catalysed: 
Release of a C-terminal amino acid, but little or no action with -Asp, -Glu, -Arg, -Lys or -Pro.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133048 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Carboxypeptidase A1 Chain: A
Molecule details ›
Chain: A
Length: 309 amino acids
Theoretical weight: 34.72 KDa
Source organism: Bos taurus
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P00730 (Residues: 111-419; Coverage: 77%)
Gene names: CPA, CPA1
Sequence domains: Zinc carboxypeptidase
Structure domains: Zn peptidases
Carboxypeptidase inhibitor Chain: B
Molecule details ›
Chain: B
Length: 75 amino acids
Theoretical weight: 7.95 KDa
Source organism: Rhipicephalus bursa
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q5EPH2 (Residues: 23-97; Coverage: 100%)
Sequence domains: Carboxypeptidase inhibitor I68
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 5 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21
Unit cell:
a: 47.07Å b: 68.6Å c: 57.89Å
α: 90° β: 104.72° γ: 90°
R-values:
R R work R free
0.16 0.154 0.185
Expression systems:
  • Komagataella pastoris
  • Escherichia coli BL21

Quick links

Citations

7 review citations

The role of saliva in tick feeding.
Francischetti et al. (2009)
6 more

6 mentions without citation

Mammalian metallopeptidase inhibition at the defense barrier of Ascaris parasite.
Sanglas et al. (2009)
5 more