› 1zrh

X-ray diffraction
2.1Å resolution

Crystal structure of Human heparan sulfate glucosamine 3-O-sulfotransferase 1 in complex with PAP

Released:
DOI: 10.2210/pdb1zrh/pdb
PDB entry 1zrh coloured by chain, front view.
PDB entry 1zrh coloured by chain, side view.
PDB entry 1zrh coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Dong A, Dombrovski L, Loppnau P, Sundstrom M, Arrowsmith CH, Edwards AM, Bochkarev A, Plotnikov AN, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127147 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heparan sulfate glucosamine 3-O-sulfotransferase 1 Chain: A
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 32.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O14792 (Residues: 36-307; Coverage: 95%)
Gene names: 3OST, 3OST1, HS3ST1
Sequence domains: Sulfotransferase domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand A3P 1 x A3P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E
Spacegroup: P21212
Unit cell:
a: 58.887Å b: 135.255Å c: 48.21Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.184 0.209
Expression system: Escherichia coli BL21(DE3)

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