› 1zze

X-ray diffraction
1.8Å resolution

X-ray Structure of NADPH-dependent Carbonyl Reductase from Sporobolomyces salmonicolor

Released:
DOI: 10.2210/pdb1zze/pdb
PDB entry 1zze coloured by chain, front view.
PDB entry 1zze coloured by chain, side view.
PDB entry 1zze coloured by chain, top view.
Source organism: Sporidiobolus salmonicolor
Primary publication:
X-ray structures of NADPH-dependent carbonyl reductase from Sporobolomyces salmonicolor provide insights into stereoselective reductions of carbonyl compounds.
Kamitori S, Iguchi A, Ohtaki A, Yamada M, Kita K
J Mol Biol 352 551-8 (2005)
PMID: 16095619

Function and Biology Details

Reaction catalysed: 
An alcohol + NADP(+) = an aldehyde + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194106 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aldehyde reductase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 342 amino acids
Theoretical weight: 37.23 KDa
Source organism: Sporidiobolus salmonicolor
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UUN9 (Residues: 2-343; Coverage: 100%)
Sequence domains: NAD dependent epimerase/dehydratase family
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

1 bound ligand:
Ligand SO4 10 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P212121
Unit cell:
a: 54.919Å b: 83.444Å c: 148.262Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.193 0.233
Expression system: Escherichia coli

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Citations

3 review citations

Understanding the tolerance of the industrial yeast Saccharomyces cerevisiae against a major class of toxic aldehyde compounds.
Liu ZL. (2018)
2 more