› 2e5b

X-ray diffraction
2Å resolution

Crystal structure of Human NMPRTase as free-form

Released:
DOI: 10.2210/pdb2e5b/pdb
PDB entry 2e5b coloured by chain, front view.
PDB entry 2e5b coloured by chain, side view.
PDB entry 2e5b coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and reaction mechanism of human nicotinamide phosphoribosyltransferase.
Takahashi R, Nakamura S, Nakazawa T, Minoura K, Yoshida T, Nishi Y, Kobayashi Y, Ohkubo T
J Biochem 147 95-107 (2010)
PMID: 19819904

Function and Biology Details

Reaction catalysed: 
Nicotinamide D-ribonucleotide + diphosphate = nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155082 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nicotinamide phosphoribosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 499 amino acids
Theoretical weight: 56.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P43490 (Residues: 1-491; Coverage: 100%)
Gene names: NAMPT, PBEF, PBEF1
Sequence domains:
Structure domains: Aldolase class I

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P21
Unit cell:
a: 60.564Å b: 106.4Å c: 82.781Å
α: 90° β: 96.5° γ: 90°
R-values:
R R work R free
0.185 0.183 0.219
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Phosphoribosyl Diphosphate (PRPP): Biosynthesis, Enzymology, Utilization, and Metabolic Significance.
Hove-Jensen et al. (2017)
4 more

2 mentions without citation

Discovery of small-molecule activators of nicotinamide phosphoribosyltransferase (NAMPT) and their preclinical neuroprotective activity.
Yao et al. (2022)
1 more