› 2fh9

X-ray diffraction
2.8Å resolution

Structure and dimerization of the kinase domain from yeast Snf1

Released:
DOI: 10.2210/pdb2fh9/pdb
PDB entry 2fh9 coloured by chain, front view.
PDB entry 2fh9 coloured by chain, side view.
PDB entry 2fh9 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure and dimerization of the kinase domain from yeast Snf1, a member of the Snf1/AMPK protein family.
Nayak V, Zhao K, Wyce A, Schwartz MF, Lo WS, Berger SL, Marmorstein R
Structure 14 477-85 (2006)
PMID: 16531232

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-529816 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carbon catabolite-derepressing protein kinase Chain: A
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 31.26 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P06782 (Residues: 46-319; Coverage: 43%)
Gene names: CAT1, CCR1, D8035.20, GLC2, PAS14, SNF1, YDR477W
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P4212
Unit cell:
a: 108.677Å b: 108.677Å c: 61.62Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.345 0.215 0.26
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

19 review citations

AMP-activated/SNF1 protein kinases: conserved guardians of cellular energy.
Hardie DG. (2007)
18 more

4 mentions without citation

Substrate and docking interactions in serine/threonine protein kinases.
Goldsmith et al. (2007)
3 more