› 2a48

X-ray diffraction
2Å resolution

Crystal structure of amFP486 E150Q

Released:
DOI: 10.2210/pdb2a48/pdb
PDB entry 2a48 coloured by chain, front view.
PDB entry 2a48 coloured by chain, side view.
PDB entry 2a48 coloured by chain, top view.
Source organism: Anemonia manjano
Primary publication:
Crystal structures and mutational analysis of amFP486, a cyan fluorescent protein from Anemonia majano.
Henderson JN, Remington SJ
Proc Natl Acad Sci U S A 102 12712-7 (2005)
PMID: 16120682

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193620 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GFP-like fluorescent chromoprotein amFP486 Chain: A
Molecule details ›
Chain: A
Length: 238 amino acids
Theoretical weight: 26.6 KDa
Source organism: Anemonia manjano
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9U6Y6 (Residues: 2-229; Coverage: 100%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand BME 3 x BME
1 modified residue:
Ligand CR7 1 x CR7

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: I4122
Unit cell:
a: 113.888Å b: 113.888Å c: 82.625Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.18 0.249
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Fluorescent proteins and their applications in imaging living cells and tissues.
Chudakov et al. (2010)
13 more

1 mention without citation

Crystal structures and mutational analysis of amFP486, a cyan fluorescent protein from Anemonia majano.
Henderson et al. (2005)