› 2a4v

X-ray diffraction
1.8Å resolution

Crystal Structure of a truncated mutant of yeast nuclear thiol peroxidase

Released:
DOI: 10.2210/pdb2a4v/pdb
PDB entry 2a4v coloured by chain, front view.
PDB entry 2a4v coloured by chain, side view.
PDB entry 2a4v coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Crystal structure of the C107S/C112S mutant of yeast nuclear 2-Cys peroxiredoxin.
Choi J, Choi S, Chon JK, Choi J, Cha MK, Kim IH, Shin W
Proteins 61 1146-9 (2005)
PMID: 16245326

Function and Biology Details

Reaction catalysed: 
Thioredoxin + ROOH = thioredoxin disulfide + H(2)O + ROH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154140 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peroxiredoxin DOT5 Chain: A
Molecule details ›
Chain: A
Length: 159 amino acids
Theoretical weight: 17.86 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P40553 (Residues: 57-215; Coverage: 74%)
Gene names: DOT5, YIL010W
Sequence domains: AhpC/TSA family
Structure domains: Glutaredoxin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 6B
Spacegroup: P32
Unit cell:
a: 37.535Å b: 37.535Å c: 83.256Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.167 0.165 0.215
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Protein-protein interactions within peroxiredoxin systems.
Noguera-Mazon et al. (2006)
6 other articles

2 mentions without citation

Structure-based insights into the catalytic power and conformational dexterity of peroxiredoxins.
Hall et al. (2011)
1 more