› 2a9n

X-ray diffraction
3Å resolution

A Mutation Designed to Alter Crystal Packing Permits Structural Analysis of a Tight-binding Fluorescein-scFv complex

Released:
DOI: 10.2210/pdb2a9n/pdb
PDB entry 2a9n coloured by chain, front view.
PDB entry 2a9n coloured by chain, side view.
PDB entry 2a9n coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
A mutation designed to alter crystal packing permits structural analysis of a tight-binding fluorescein-scFv complex.
Honegger A, Spinelli S, Cambillau C, Plückthun A
Protein Sci 14 2537-49 (2005)
PMID: 16195545

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-242691 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
fluorescein-scfv Chains: A, B
Molecule details ›
Chains: A, B
Length: 264 amino acids
Theoretical weight: 27.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand ORE 2 x ORE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21212
Unit cell:
a: 81.382Å b: 100.46Å c: 61.749Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.217 0.266
Expression system: Escherichia coli

Quick links

Citations

11 citation in other articles

Structure and function of nanoparticle-protein conjugates.
Aubin-Tam et al. (2008)
10 more

4 mentions without citation

A Two-Step Approach for the Design and Generation of Nanobodies.
Wagner et al. (2018)
3 more