› 2ab4

X-ray diffraction
2.4Å resolution

Dissecting the Roles of a Strictly Conserved Tyrosine in Substrate Recognition and Catalysis by Pseudouridine 55 Synthase

Released:
DOI: 10.2210/pdb2ab4/pdb
PDB entry 2ab4 coloured by chain, front view.
PDB entry 2ab4 coloured by chain, side view.
PDB entry 2ab4 coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
Dissecting the roles of a strictly conserved tyrosine in substrate recognition and catalysis by pseudouridine 55 synthase.
Phannachet K, Elias Y, Huang RH
Biochemistry 44 15488-94 (2005)
PMID: 16300397

Function and Biology Details

Reaction catalysed: 
tRNA uridine(55) = tRNA pseudouridine(55)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194647 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
tRNA pseudouridine synthase B Chain: A
Molecule details ›
Chain: A
Length: 309 amino acids
Theoretical weight: 35.53 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9WZW0 (Residues: 1-309; Coverage: 100%)
Gene names: TM_0856, truB
Sequence domains:
Structure domains:
5'-R(*CP*CP*AP*CP*GP*GP*UP*(FHU)P*CP*GP*AP*AP*UP*CP*CP*GP*UP*GP*GP*C)-3' Chain: B
Molecule details ›
Chain: B
Length: 20 nucleotides
Theoretical weight: 6.41 KDa
Source organism: Thermotoga maritima
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
1 modified residue:
Ligand FHU 1 x FHU

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P21212
Unit cell:
a: 157.87Å b: 51.87Å c: 57.15Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.222 0.272
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

10 review citations

Pseudouridine synthases.
Hamma et al. (2006)
9 more

1 mention without citation

Computational approaches to 3D modeling of RNA.
Laing et al. (2010)