› 2ada

X-ray diffraction
2.4Å resolution

ATOMIC STRUCTURE OF ADENOSINE DEAMINASE COMPLEXED WITH A TRANSITION-STATE ANALOG: UNDERSTANDING CATALYSIS AND IMMUNODEFICIENCY MUTATIONS

Released:
DOI: 10.2210/pdb2ada/pdb
PDB entry 2ada coloured by chain, front view.
PDB entry 2ada coloured by chain, side view.
PDB entry 2ada coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Atomic structure of adenosine deaminase complexed with a transition-state analog: understanding catalysis and immunodeficiency mutations.
Wilson DK, Rudolph FB, Quiocho FA
Science 252 1278-84 (1991)
PMID: 1925539

Function and Biology Details

Reaction catalysed: 
2'-deoxyadenosine + H(2)O = 2'-deoxyinosine + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137300 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenosine deaminase Chain: A
Molecule details ›
Chain: A
Length: 352 amino acids
Theoretical weight: 40.05 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P03958 (Residues: 1-352; Coverage: 100%)
Gene name: Ada
Sequence domains: Adenosine deaminase
Structure domains: Metal-dependent hydrolases

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand HPR 1 x HPR
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2
Unit cell:
a: 102.36Å b: 94.11Å c: 72.93Å
α: 90° β: 127.19° γ: 90°
R-values:
R R work R free
0.195 0.195 not available
Expression system: Escherichia coli

Quick links

Citations

39 review citations

Calcineurin: form and function.
Rusnak et al. (2000)
38 more

10 mentions without citation

Towards the development of universal, fast and highly accurate docking/scoring methods: a long way to go.
Moitessier et al. (2008)
9 more