› 2b04

X-ray diffraction
2.5Å resolution

Crystal Structure of Porcine Pancreatic Phospholipase A2 in Complex with Glycochenodeoxycholate

Released:
DOI: 10.2210/pdb2b04/pdb
PDB entry 2b04 coloured by chain, front view.
PDB entry 2b04 coloured by chain, side view.
PDB entry 2b04 coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
Structural basis for bile salt inhibition of pancreatic phospholipase A2.
Pan YH, Bahnson BJ
J Mol Biol 369 439-50 (2007)
PMID: 17434532

Function and Biology Details

Reaction catalysed: 
Phosphatidylcholine + H(2)O = 1-acylglycerophosphocholine + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-132748 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phospholipase A2, major isoenzyme Chain: A
Molecule details ›
Chain: A
Length: 124 amino acids
Theoretical weight: 14.01 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli
UniProt:
  • Canonical: P00592 (Residues: 23-146; Coverage: 95%)
Gene name: PLA2G1B
Sequence domains: Phospholipase A2
Structure domains: Phospholipase A2 domain

Ligands and Environments

3 bound ligands:
Ligand CHO 1 x CHO
Ligand CA 3 x CA
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P3121
Unit cell:
a: 69.156Å b: 69.156Å c: 67.043Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.219 0.211 0.244
Expression system: Escherichia coli

Quick links

Citations

10 citation in other articles

Bile acids cause secretory phospholipase A2 activity enhancement, revertible by exogenous surfactant administration.
De Luca et al. (2009)
9 more

11 mentions without citation

Potent Molecular Feature-based Neutralizing Monoclonal Antibodies as Promising Therapeutics Against SARS-CoV-2 Infection.
Kombe Kombe et al. (2021)
10 more