› 2b4r

X-ray diffraction
2.25Å resolution

Crystal structure of glyceraldehyde-3-phosphate dehydrogenase from Plasmodium falciparum at 2.25 Angstrom Resolution reveals intriguing extra electron density in the active site

Released:
DOI: 10.2210/pdb2b4r/pdb
PDB entry 2b4r coloured by chain, front view.
PDB entry 2b4r coloured by chain, side view.
PDB entry 2b4r coloured by chain, top view.
Source organism: Plasmodium falciparum
Primary publication:
Crystal structure of glyceraldehyde-3-phosphate dehydrogenase from Plasmodium falciparum at 2.25 A resolution reveals intriguing extra electron density in the active site.
Robien MA, Bosch J, Buckner FS, Van Voorhis WC, Worthey EA, Myler P, Mehlin C, Boni EE, Kalyuzhniy O, Anderson L, Lauricella A, Gulde S, Luft JR, DeTitta G, Caruthers JM, Hodgson KO, Soltis M, Zucker F, Verlinde CL, Merritt EA, Schoenfeld LW, Hol WG
Proteins 62 570-7 (2006)
PMID: 16345073

Function and Biology Details

Reaction catalysed: 
D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186130 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glyceraldehyde-3-phosphate dehydrogenase Chains: O, P, Q, R
Molecule details ›
Chains: O, P, Q, R
Length: 345 amino acids
Theoretical weight: 37.64 KDa
Source organism: Plasmodium falciparum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8T6B1 (Residues: 1-337; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
2 bound ligands:
Ligand AES 3 x AES
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21
Unit cell:
a: 69.327Å b: 104.585Å c: 90.838Å
α: 90° β: 107.35° γ: 90°
R-values:
R R work R free
0.186 0.183 0.243
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Structural genomics and drug discovery: all in the family.
Weigelt et al. (2008)
1 more

8 mentions without citation

The apicomplexan glideosome and adhesins - Structures and function.
Boucher et al. (2015)
7 more