› 2bax

X-ray diffraction
1.1Å resolution

Atomic Resolution Structure of the Double Mutant (K53,56M) of Bovine Pancreatic Phospholipase A2

Released:
DOI: 10.2210/pdb2bax/pdb
PDB entry 2bax coloured by chain, front view.
PDB entry 2bax coloured by chain, side view.
PDB entry 2bax coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Atomic resolution (0.97 A) structure of the triple mutant (K53,56,121M) of bovine pancreatic phospholipase A2.
Sekar K, Rajakannan V, Gayathri D, Velmurugan D, Poi MJ, Dauter M, Dauter Z, Tsai MD
Acta Crystallogr Sect F Struct Biol Cryst Commun 61 3-7 (2005)
PMID: 16508077

Function and Biology Details

Reaction catalysed: 
Phosphatidylcholine + H(2)O = 1-acylglycerophosphocholine + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132750 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phospholipase A2 Chain: A
Molecule details ›
Chain: A
Length: 123 amino acids
Theoretical weight: 13.81 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00593 (Residues: 23-145; Coverage: 95%)
Gene name: PLA2G1B
Sequence domains: Phospholipase A2
Structure domains: Phospholipase A2 domain

Ligands and Environments

4 bound ligands:
Ligand CA 1 x CA
Ligand CL 1 x CL
Ligand MRD 4 x MRD
Ligand MPD 1 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: P3121
Unit cell:
a: 46.057Å b: 46.057Å c: 101.138Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.119 0.119 0.156
Expression system: Escherichia coli

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Citations

2 mentions without citation

Expression, functional, and structural analysis of proteins critical for otoconia development.
Xu et al. (2010)
1 more