› 2bec

X-ray diffraction
2.7Å resolution

Crystal structure of CHP2 in complex with its binding region in NHE1 and insights into the mechanism of pH regulation

Released:
DOI: 10.2210/pdb2bec/pdb
PDB entry 2bec coloured by chain, front view.
PDB entry 2bec coloured by chain, side view.
PDB entry 2bec coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of CHP2 complexed with NHE1-cytosolic region and an implication for pH regulation.
Ammar YB, Takeda S, Hisamitsu T, Mori H, Wakabayashi S
EMBO J 25 2315-25 (2006)
PMID: 16710297

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129100 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calcineurin B homologous protein 2 Chain: A
Molecule details ›
Chain: A
Length: 202 amino acids
Theoretical weight: 23.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O43745 (Residues: 1-196; Coverage: 100%)
Gene names: CHP2, HCA520
Sequence domains:
Structure domains: EF-hand
Sodium/hydrogen exchanger 1 Chain: B
Molecule details ›
Chain: B
Length: 43 amino acids
Theoretical weight: 5.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P19634 (Residues: 503-545; Coverage: 5%)
Gene names: APNH1, NHE1, SLC9A1

Ligands and Environments

1 bound ligand:
Ligand YT3 2 x YT3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU, SPRING-8 BEAMLINE BL44B2
Spacegroup: P43
Unit cell:
a: 49.262Å b: 49.262Å c: 102.587Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.218 0.287
Expression system: Escherichia coli

Quick links

Citations

14 review citations

SLC9/NHE gene family, a plasma membrane and organellar family of Na⁺/H⁺ exchangers.
Donowitz et al. (2013)
13 more

5 mentions without citation

The structure of the C-terminal domain of the protein kinase AtSOS2 bound to the calcium sensor AtSOS3.
Sánchez-Barrena et al. (2007)
4 more