› 2bm6

X-ray diffraction
2.2Å resolution

The Structure of MfpA (Rv3361c, C2221 Crystal form). The Pentapeptide Repeat Protein from Mycobacterium tuberculosis Folds as A Right- handed Quadrilateral Beta-helix.

Released:
DOI: 10.2210/pdb2bm6/pdb
PDB entry 2bm6 coloured by chain, front view.
PDB entry 2bm6 coloured by chain, side view.
PDB entry 2bm6 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
A fluoroquinolone resistance protein from Mycobacterium tuberculosis that mimics DNA.
Hegde SS, Vetting MW, Roderick SL, Mitchenall LA, Maxwell A, Takiff HE, Blanchard JS
Science 308 1480-3 (2005)
PMID: 15933203

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129324 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pentapeptide repeat family protein Chain: A
Molecule details ›
Chain: A
Length: 186 amino acids
Theoretical weight: 20.33 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: O50390 (Residues: 1-183; Coverage: 100%)
Gene name: MT3469
Sequence domains:
Structure domains: E3 ubiquitin-protein ligase SopA

Ligands and Environments

1 bound ligand:
Ligand CS 6 x CS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: C2221
Unit cell:
a: 33.393Å b: 48.573Å c: 188.367Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.197 0.256
Expression system: Escherichia coli

Quick links

Citations

58 review citations

Persister cells, dormancy and infectious disease.
Lewis K. (2007)
57 more

1 mention without citation

Mechanisms of drug resistance: quinolone resistance.
Hooper et al. (2015)