› 2bmf

X-ray diffraction
2.41Å resolution

Dengue virus RNA helicase at 2.4A

Released:
DOI: 10.2210/pdb2bmf/pdb
PDB entry 2bmf coloured by chain, front view.
PDB entry 2bmf coloured by chain, side view.
PDB entry 2bmf coloured by chain, top view.
Source organism: Dengue virus 2
Primary publication:
Structure of the Dengue virus helicase/nucleoside triphosphatase catalytic domain at a resolution of 2.4 A.
Xu T, Sampath A, Chao A, Wen D, Nanao M, Chene P, Vasudevan SG, Lescar J
J Virol 79 10278-88 (2005)
PMID: 16051821

Function and Biology Details

Reactions catalysed: 
Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187513 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA HELICASE Chains: A, B
Molecule details ›
Chains: A, B
Length: 451 amino acids
Theoretical weight: 51.63 KDa
Source organism: Dengue virus 2
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q91H74 (Residues: 1646-2093; Coverage: 13%)
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 54.9Å b: 178.1Å c: 55.4Å
α: 90° β: 101.3° γ: 90°
R-values:
R R work R free
0.222 0.222 0.27
Expression system: Escherichia coli BL21

Quick links

Citations

35 review citations

Structural proteomics of dengue virus.
Perera et al. (2008)
34 more

23 mentions without citation

Focus on flaviviruses: current and future drug targets.
Geiss et al. (2009)
22 more